Where: At the Research Institute of the McGill University Health Centre (RI‑MUHC) Glen Site, block E, room M1.3509, 1001 Decarie Boulevard, Montreal, Quebec H4A 3J1
When: Wednesday, March 13, 12 p.m.
Speaker: Mike Tyers, PhD
Principal Investigator, Institute for Research in Immunology and Cancer
Professor, Department of Medicine, University of Montreal
Charting the genetic interaction landscape of human cells through genome-wide CRISPR screens
The phenotype of every living system is governed by a vast landscape of genetic interactions. Recent CRISPR-based strategies have enabled systematic genetic screens to map genotype-phenotype relationships in human cells. I will describe how fitness defects uncovered in genomewide CRISPR screens correlate with different features of the human proteome. The contextual specificity of genotype-phenotype relationships can be revealed by system perturbation, and to this end we have undertaken CRISPR screens to build genetic interaction profiles for hundreds of drugs, toxins and other bioactive agents. This extensive chemical-gene interaction matrix reveals on- and off-target drug effects, identifies mechanisms of drug resistance and sensitivity, and illuminates the underlying genetic network architecture of the cell. The chemicalgenetic landscape will inform new network-based therapeutic strategies against many diseases.