CFTR • chloride secretion • cystic fibrosis • electrophysiology • epithelial transport • fluorescence imaging • ion channel function • ion channel structure
My research focuses on cystic fibrosis and other diseases that affect epithelial tissues. We study the cellular and molecular physiology of transepithelial salt and water transport, explore the regulation of CFTR and other ion channels, examine the cell biology of CFTR, especially its trafficking, and work with partners to develop new therapeutics for cystic fibrosis.
Billet A, Luo Y, Balghi H, Hanrahan JW 2013 Role of Tyrosine Phosphorylation in the Muscarinic Activation of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). J. Biol. Chem. 288(30): 21815-23.
Lesimple P, Goepp J, Palmer ML, Fahrenkrug SC, O'Grady SM, Ferraro P, Robert R, Hanrahan JW 2013 CFTR is Expressed in Mucin Granules from Calu-3 and Primary Human Airway Epithelial Cells. Am. J. Respir. Cell. Mol. Biol. June 6 (Epub ahead of print] PMID: 23742042.
Shan J, Liao J, Huang J, Robert R, Palmer ML, Fahrenkrug SC, O'Grady SM, Hanrahan JW 2012. Bicarbonate-dependent chloride transport drives fluid secretion by the human airway epithelial cell line Calu-3. J Physiol. 590(Pt 21):5273-97.
Balghi H, Robert R, Rappaz B, Zhang X, Wohlhuter-Haddad A, Evagelidis A, Luo Y, Goepp J, Ferraro P, Roméo P, Trebak M, Wiseman PW, Thomas DY, Hanrahan JW 2011. Enhanced Ca2+ entry due to Orai1 plasma membrane insertion increases IL-8 secretion by cystic fibrosis airways. FASEB J. 25(12):4274-91.
Robert R, Carlile GW, Liao J, Balghi H, LeSimple P, Liu N, Kus B, Rotin D, Wilke M, De Jonge HR, Scholte BJ, Thomas DY, Hanrahan JW 2010. Correction of the Delta phe508 cystic fibrosis transmembrane conductance regulator trafficking defect by the bioavailable compound glafenine. Mol. Pharmacol. 77:922-930.