Tuberculosis (TB) • Mycobacterium tuberculosis • molecular microbiology • bacterial pathogenesis
My research focuses on molecular aspects of the pathogenesis of Mycobacterium tuberculosis, the bacterial agent responsible for the infectious disease, tuberculosis (TB). Despite the existence of effective antibacterial drugs and a partially effective vaccine for more than half a century, TB still remains the cause of two million deaths worldwide each year. Although early studies considered TB strains to be restricted in terms of their genetic and antigenic variability, more recent evidence indicates that M. tuberculosis has evolved into several genetically diverse lineages of strains that are likely to possess unique attributes related to the transmission and development of disease. My laboratory utilizes a combination of microbial genetics, biochemistry and whole animal (mouse) in vivo infection studies to investigate the array of metabolic and virulence strategies available to this phenotypically diverse pathogen. Increasing our knowledge and understanding of this diversity will greatly enhance future efforts aimed at developing new strategies to successfully diagnose and treat TB disease.
Domenech P, Rog A, Moolji J, Radomski N, Fallow A, Leon-Solis L, Bowes J, Behr MA & Reed MB (2014). The origins of a 350-kilobase genomic duplication in Mycobacterium tuberculosis and its impact on virulence. Infection and Immunity 82: 2902-2912. PMID: 24778110.
Hansen JM, Golchin SA, Veyrier FJ, Domenech P, Boneca IG, Azad AK, Rajaram MV, Schlesinger LS, Divangahi M, Reed MB & Behr MA (2014). N-glycolylated peptidoglycan contributes to the immunogenicity but not pathogenicity of Mycobacterium tuberculosis. The Journal of Infectious Diseases 209: 1045-1054. PMID: 24265438.
Domenech P, Kolly GS, Leon-Solis L, Fallow A & Reed MB (2010). Massive gene duplication event among clinical isolates of the Mycobacterium tuberculosis W/Beijing family. Journal of Bacteriology 192: 4562-4570. PMID: 20639330.
Fallow A, Domenech P & Reed MB (2010). Strains of the East Asian (W/Beijing) lineage of Mycobacterium tuberculosis are DosS/DosT-DosR two-component regulatory system natural mutants. Journal of Bacteriology 192: 2228-2238. PMID: 20154135.
Domenech P & Reed MB (2009). Rapid and spontaneous loss of phthiocerol dimycocerosate (PDIM) from Mycobacterium tuberculosis grown in vitro: implications for virulence studies. Microbiology 155: 3532-3543. PMID: 19661177.