pathogenic fungi • transcription factors • drug resistance • genome-wide analysis
My research focuses on functional genomics in budding yeast and the human fungal pathogen Candida glabrata. We are focusing on the Gal4 family of transcriptional regulators. The function of many of these Gal4 members is unknown or poorly defined. To better understand the role of these transcriptional regulators and identify their target genes, we are using various approaches including genetics, molecular biology and whole-genome analysis of gene expression with DNA microarrays. We are also determining the role of related Gal4 members in conferring resistance to antifungal drugs in the human pathogen Candida glabrata.
Klimova, N., N. Kachurina, R. Yeung and B Turcotte (2014) Phenotypic analysis of a family of transcriptional regulators, the zinc cluster proteins, in the human fungal pathogen Candida glabrata G3: Genes, Genomes and Genet. 4: 931-940. PMID: 24657903.
Gasmi N, P.E. Jacques, N. Klimova, X. Guo, A. Ricciardi, F. Robert and B. Turcotte (2014) The switch from fermentation to respiration in Saccharomyces cerevisiae is regulated by the Ert1 transcriptional activator/repressor. Genetics 198: 547-560. PMID: 25123508.
Shah, Z, R. Mahruba and B. Turcotte (2013). The anti-cancer drug tirapazamine has antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Clostridium difficile. FEMS Microb. Lett. 347: 61-69. PMID: 23888874.
Clarke et al. (2013) Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of pattern recognition and tyrosine kinase signaling. Genome Biology 14: R11. PMID: 23375108.
Soontorngun, N., S Baramee, C. Tangsombatvichit, P. Thepnok, S. Cheevadhanarak, F. Robert; and B. Turcotte (2012). Genome-wide location analysis reveals an important overlap between the targets of the yeast transcriptional regulators Rds2 and Adr1. Biochem. Biophys. Res. Comm. 423: 632-7. PMID: 22687600.