Hugh Clarke, PhD
reproduction • infertility • oocyte • follicle
My research focuses on the growth and development of the oocyte within the follicle of the ovary before it is ovulated and fertilized to produce a new individual. Growth is a crucial period during the development of the oocyte, because this is when it manufactures and stores molecules that the embryo will need after fertilization. Because growth is a very slow process, it is also a time when the oocyte is vulnerable to damage from environmental toxins and this damage can affect the health of the embryo. We are particularly interested in how the developing oocyte communicates with the surrounding cells of the follicle to ensure its healthy development.
El-Hayek S, Clarke HJ. Follicle-Stimulating Hormone Increases Gap Junctional Communication Between the Somatic and Germ-Line Follicular Compartments During Murine Oogenesis. Biol Reprod. 2015 Jun 10. pii: biolreprod.115.129569. [Epub ahead of print] PMID: 26063870.
El-Hayek S, Demeestere I, Clarke HJ. Follicle-stimulating hormone regulates expression and activity of epidermal growth factor receptor in the murine ovarian follicle. Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16778-83. doi: 10.1073/pnas.1414648111. Epub 2014 Nov 10. PMID: 25385589.
Edwards N, Farookhi R, Clarke HJ. Identification of a &beta-galactosidase transgene that provides a live-cell marker of transcriptional activity in growing oocytes and embryos. Mol Hum Reprod. 2015 Jul;21(7):583-93. doi: 10.1093/molehr/gav020. Epub 2015 Apr 16. PMID: 25882542.
Clarke HJ, Vieux KF. Epigenetic inheritance through the female germ-line: The known, the unknown, and the possible. Semin Cell Dev Biol. 2015 Jul 13. pii: S1084-9521(15)00130-5. doi: 10.1016/j.semcdb.2015.07.003. [Epub ahead of print] PMID: 26183189.
Clarke HJ. Post-transcriptional control of gene expression during mouse oogenesis. Results Probl Cell Differ. 2012;55:1-21. doi: 10.1007/978-3-642-30406-4_1. Review. PMID: 22918798.