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John Hanrahan, PhD

Research Institute of the McGill University Health Centre

Regular Member
Respiratory Health


McGill University Health Centre (MUHC)

Researcher


McGill University

Professor, Department of Physiology


Research Interest


My research focuses on cystic fibrosis and other diseases that affect epithelial tissues. We study the cellular and molecular physiology of transepithelial salt and water transport, explore the regulation of CFTR and other ion channels, examine the cell biology of CFTR, especially its trafficking, and work with partners to develop new therapeutics for cystic fibrosis.

Research Foci


  • structure-function studies of the CFTR channel using molecular biology, patch clamping, and imaging of fluorescent and other tagged constructs
  • identification and characterization of compounds that correct the trafficking of misfolded CFTR and activate chloride channels using high throughput screening, western blotting, electrophysiology and transgenic mice
  • bicarbonate secretion and its role in host defense using epithelial cell cultures and bacterial killing assays

Keywords


CFTR, chloride secretion, cystic fibrosis, electrophysiology, epithelial transport, fluorescence imaging, ion channel function, ion channel structure

Selected Publications


Click on Pubmed to see my current publications list

  • Billet A, Luo Y, Balghi H, Hanrahan JW 2013 Role of Tyrosine Phosphorylation in the Muscarinic Activation of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). J. Biol. Chem. 288(30): 21815-23.

  • Lesimple P, Goepp J, Palmer ML, Fahrenkrug SC, O'Grady SM, Ferraro P, Robert R, Hanrahan JW 2013 CFTR is Expressed in Mucin Granules from Calu-3 and Primary Human Airway Epithelial Cells. Am. J. Respir. Cell. Mol. Biol. June 6 (Epub ahead of print] PMID: 23742042.

  • Shan J, Liao J, Huang J, Robert R, Palmer ML, Fahrenkrug SC, O'Grady SM, Hanrahan JW 2012. Bicarbonate-dependent chloride transport drives fluid secretion by the human airway epithelial cell line Calu-3. J Physiol. 590(Pt 21):5273-97.

  • Balghi H, Robert R, Rappaz B, Zhang X, Wohlhuter-Haddad A, Evagelidis A, Luo Y, Goepp J, Ferraro P, Roméo P, Trebak M, Wiseman PW, Thomas DY, Hanrahan JW 2011. Enhanced Ca2+ entry due to Orai1 plasma membrane insertion increases IL-8 secretion by cystic fibrosis airways. FASEB J. 25(12):4274-91.

  • Robert R, Carlile GW, Liao J, Balghi H, LeSimple P, Liu N, Kus B, Rotin D, Wilke M, De Jonge HR, Scholte BJ, Thomas DY, Hanrahan JW 2010. Correction of the Delta phe508 cystic fibrosis transmembrane conductance regulator trafficking defect by the bioavailable compound glafenine. Mol. Pharmacol. 77:922-930.