What we can do for you

Flow cytometry

Purpose:
Measuring the relative fluorescence intensity of particles

What is flow cytometry?

Applications include:
  • Immunophenotyping
  • Fluorescent proteins
  • Proliferation
  • Cytokine detection
  • Phosphorylation
  • Apopotosis
  • Cell cycle
Equipment:
 

Cell sorting

Purpose:
  • Enrichment
  • Purification
  • Cloning

What is cell sorting?

Applications include:
  • In vitro experiment
  • Adoptive transfer
  • RNA extraction
Equipment:
     

Imaging flow cytometry

Purpose:
Measuring the relative fluorescence intensity and taking microscopic images of cells

What is imaging flow cytometry?

Applications include:
  • Cell signalling
  • Cell cycle and mitosis
  • Internalization
  • Colocalization
  • Cell-cell interaction
  • Morphology
  • Cell death and autophagy
  • Spot counting
Equipment:
 

Other services:

Automated cell separation Flow cytometry in the Containment Level 3 (CL3) facility

Consultation

Experimental design

Training

Data analysis


Access to the Immunophenotyping Platform is managed via the web-based iLab Solution's Core Facility Management software. Investigators must sign up for an iLab account to request core services or to schedule equipment or training.

Click here to access our iLab website.

Flow cytometry and imaging flow cytometry services are available with or without assistance. Training is mandatory prior to using the equipment without assistance.

Cell sorters are operated by platform staff only. Please contact the platform at least two weeks before the planned sort date in order to schedule time on the instrument.

The cost of services and equipment usage will vary according to specific study requirements. Click here for the Platform pricing list.

Data analysis
Platform users have access to a computer for analysis. This computer is equipped with FlowJo, a well-known data analysis program. A one-hour training course on this program is available.

Flow cytometry in the Containment Level 3 (CL3) Platform
Satellite flow cytometry instruments are available in each pod of the CL3 facility. For more information, please contact the platformYou must also contact the CL3 Platform to get access to this facilty.

For additional information, you may contact the Immunophenotyping Platform manager:

Marie-Hélène Lacombe
Tel: 514-934-1934 ext. 76352 
Email: immunophenotyping.rimuhc@mcgill.ca