The Molecular Imaging Platform is one of the biggest imaging centres in Eastern Canada. The state-of-the-art light microscopy facility specializes in cell, tissue and intravital imaging. The platform houses multiple diversified and sophisticated systems, including epifluorescent microscopes for basic applications and live cell imaging (Ca2+ and ratiometric imaging); confocal microscopes for 2D and 3D imaging, fluorescence correlation spectroscopy (FCS), super-resolution imaging (SIM, PALM and dSTORM) and live cell imaging with photo manipulation. The platform has the first multi-photon microscope with dual IR-OPO lasers for intravital imaging in Canada. Other instruments include high-content screening/imaging (HCS/HCI) microscopes for medium-size screening and imaging as well as plate readers for multimodality applications (label-free, absorbance, bioluminescence and fluorescence). The Molecular Imaging Platform also provides access to offline workstations fitted with software enabling the analysis of high quality histochemical, morphological and biophysical images. The platform's experienced staff are available for consultation and assistance on experimental design, image acquisition and the application of various cell biology methods. Training sessions for the development of protocols or data analysis as well as hands-on practice, can be offered to individuals or groups.